Evaluation of toxoplasmosis in pregnant women using dot-immunogold-silver

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Analysis of toxoplasmosis in pregnant girls utilizing dot-immunogold-silver staining with recombinant Toxoplasma gondii peroxiredoxin protein

 Background: Toxoplasma gondii an infection endangers human well being and impacts animal husbandry. Serological detection is the primary methodology used for epidemiological investigations and prognosis of toxoplasmosis. The important thing to efficient prognosis of toxoplasmosis is using a standardized antigen and a particular and delicate detection methodology. Peroxiredoxin is an antigenic protein and vaccine candidate antigen of T. gondii that has not but been exploited for diagnostic software.

Strategies: On this research, recombinant T. gondii peroxiredoxin protein (rTgPrx) was ready and utilized in dot-immunogold-silver staining (Dot-IGSS) to detect IgG antibodies in serum from mice and pregnant girls. The rTgPrx-Dot-IGSS methodology was established and optimized utilizing mouse serum. Moreover, serum samples from pregnant girls had been analyzed by rTgPrx-Dot-IGSS.

Outcomes: Forty serum samples from mice contaminated with T. gondii and twenty adverse serum samples had been analyzed. The sensitivity and specificity of rTgPrx-Dot-IGSS had been 97.5 and 100%, respectively, equal to these of a industrial ELISA package for anti-Toxoplasma IgG antibody. Moreover, 540 serum samples from pregnant girls had been screened with a industrial ELISA package. Eighty-three constructive and 60 adverse serum samples had been analyzed by rTgPrx-Dot-IGSS. The constructive price was 95.18%, akin to that obtained with the industrial ELISA package.

Conclusions: The Dot-IGSS methodology with rTgPrx as an antigen may be helpful for diagnosing T. gondii an infection in people.

The Validation of the Wheat Gluten ELISA Equipment.

Background: It is very important analyze the presence of wheat/gluten in meals to keep away from wheat allergy or celiac illness.

Goal: The Wheat/Gluten ELISA package was developed to measure complete wheat protein or gluten content material in wheat, barley, and rye cereals as uncooked supplies, and processed meals. Validation as as to if this package is appropriate for quantifying complete wheat protein/gluten was carried out.

Strategies: The Wheat/Gluten ELISA package was designed as a sandwich ELISA based mostly on antigliadin polyclonal antibody. Selectivity, interference research, matrix research together with incurred meals, robustness, stability, and lot-to-lot consistency research had been carried out for the Wheat/Gluten ELISA package. Incurred matrix research had been additionally carried out in an impartial laboratory.

Outcomes: The evaluation of 38 totally different substances revealed no cross-reactivity above the LOQ apart from oats. Recoveries of the spiked samples had been largely within the vary of 75-140%, together with an impartial laboratory outcome. The LOD of the ELISA was discovered to be 0.02-0.16 mg/kg. Robustness testing proved that extraction time and incubation time of first response and enzyme response had no important affect on quantified worth. The steadiness at 2-8°C was discovered to exceed 12 months. Good lot-to-lot consistency was noticed.

Conclusions: The Wheat/Gluten ELISA package confirmed good analytical efficiency within the quantitative evaluation of complete wheat protein/gluten within the recognized meals merchandise utilizing the AOAC Efficiency Examined Methodology(s)SM program.

Highlights: The Wheat/Gluten ELISA package was validated and confirmed good analytical efficiency within the quantitative evaluation of complete wheat protein/gluten in meals.

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Rat Cys-C (Cystatin C) ELISA Kit

EKE62085-5x96T 5x96T
EUR 2667.6

Rat Cys-C (Cystatin C) ELISA Kit

EKE62085-96T 96T
EUR 561.6

Rat Cystatin C ELISA

1042 -
EUR 567
Description: Nephrology ELISA

Cys-C ELISA Kit| Rat Cystatin C ELISA Kit

EF017675 96 Tests
EUR 826.8

Cystatin C ELISA Kit (Rat) (OKIA00140)

OKIA00140 96 Wells
EUR 756
Description: Description of target: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L. ;Species reactivity: ;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.206 ng/ml

Nori® Rat Cystatin C ELISA Kit

GR118348 96-well
EUR 461

Rat Cystatin C,Cys-C ELISA kit

E02C2122-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin C,Cys-C ELISA kit

E02C2122-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin C,Cys-C ELISA kit

E02C2122-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin C,Cys-C ELISA Kit

CN-01845R1 96T
EUR 577.2

Rat Cystatin C,Cys-C ELISA Kit

CN-01845R2 48T
EUR 398.4

Rat Cystatin C, Cys-C ELISA Kit

CSB-E08385r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Cystatin C, Cys-C ELISA Kit

1-CSB-E08385r
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-48T 48T
EUR 424.8

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-96T 96T
EUR 685.2

Rat Cystatin C(Cys-C)ELISA Kit

QY-E11499 96T
EUR 433.2

Rat Cystatin C (Cys-C) ELISA Kit

EKN44675-48T 48T
EUR 370.02

Rat Cystatin C (Cys-C) ELISA Kit

EKN44675-5x96T 5x96T
EUR 2510.85

Rat Cystatin C (Cys-C) ELISA Kit

EKN44675-96T 96T
EUR 528.6

Rat Cystatin C,Cys-C ELISA Kit

EKC38930-48T 48T
EUR 535.99

Rat Cystatin C,Cys-C ELISA Kit

EKC38930-5x96T 5x96T
EUR 3637.08

Rat Cystatin C,Cys-C ELISA Kit

EKC38930-96T 96T
EUR 765.7

Rat Cystatin C,Cys-C ELISA Kit

YLA0395RA-48T 48T
EUR 465

Rat Cystatin C,Cys-C ELISA Kit

YLA0395RA-96T 96T
EUR 600

Rat Cystatin C (Cys-C) ELISA Kit

EKU03564-48T 48T
EUR 542.78

Rat Cystatin C (Cys-C) ELISA Kit

EKU03564-5x96T 5x96T
EUR 3683.15

Rat Cystatin C (Cys-C) ELISA Kit

EKU03564-96T 96T
EUR 775.4

Rat Cystatin C,Cys-C ELISA Kit

SL0221Ra -
EUR 498

Rat Cystatin C (CST3) ELISA Kit

abx256140-96tests 96 tests
EUR 801.6

Rat Cystatin C (CST3) ELISA Kit

abx256859-96tests 96 tests
EUR 801.6

ELISA kit for Rat Cystatin-C

EK2416 96 tests
EUR 562.8
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Cystatin-C in samples from serum, plasma, tissue homogenates and other biological fluids.

Rat Cystatin A ELISA kit

E02C0350-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cystatin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin A ELISA kit

E02C0350-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cystatin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin A ELISA kit

E02C0350-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cystatin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin B ELISA Kit

ELA-E1853r 96 Tests
EUR 1063.2

Rat Cystatin 3 ELISA Kit

IRTCST3KT each
EUR 718
Description: Rat Cystatin 3 ELISA Kit

Cystatin C (CST3) (Rat) ELISA Kit

E4305-100 each
EUR 874.8

Bovine Cystatin (Cystatin) ELISA Kit

YLA0368BO-48T 48T
EUR 502.5

Bovine Cystatin (Cystatin) ELISA Kit

YLA0368BO-96T 96T
EUR 618.75

Rat Cst3/ Cystatin-C ELISA Kit

E0244Ra 1 Kit
EUR 655.2

Rat Cystatin C PicoKine ELISA Kit

EK1109 96 wells
EUR 510
Description: For quantitative detection of rat Cystatin C in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

ELISA kit for Rat Cys-C (Cystatin C)

E-EL-R0304 1 plate of 96 wells
EUR 452.4
Description: A sandwich ELISA kit for quantitative measurement of Rat Cys-C (Cystatin C) in samples from Serum, Plasma, Cell supernatant

Dog Cystatin C ELISA kit

E08C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cystatin C ELISA kit

E08C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cystatin C ELISA kit

E08C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cystatin C ELISA kit

E07C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cystatin C ELISA kit

E07C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cystatin C ELISA kit

E07C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cystatin C ELISA kit

E06C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cystatin C ELISA kit

E06C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cystatin C ELISA kit

E06C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cystatin C ELISA kit

55R-1733 1 kit
EUR 781.2
Description: ELISA kit for the detection of Cystatin C in the research laboratory

Mouse Cystatin C ELISA Kit

55R-1734 1 kit
EUR 748.8
Description: ELISA kit for detection of Cystatin C in the research laboratory

Human Cystatin C ELISA Kit

DEIA2709 96T
EUR 1051.2
Description: For the quantitative determination of human Cystatin C concentrations in cell culture supernates, serum, plasma, saliva, urine, and human milk.

Human Cystatin C ELISA kit

E01C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cystatin C ELISA kit

E01C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cystatin C ELISA kit

E01C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cystatin C ELISA kit

E03C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cystatin C ELISA kit

E03C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cystatin C ELISA kit

E03C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cystatin C ELISA kit

LF-EK50705 1×96T
EUR 777.6

Human Cystatin C ELISA kit

LF-EK50565 1×96T
EUR 777.6

Human Cystatin C ELISA Kit

IHUCSTCKT each
EUR 654
Description: Human Cystatin C ELISA Kit

Mouse Cystatin C ELISA Kit

IMSCSTCKT each
EUR 729
Description: Mouse Cystatin C ELISA Kit

Human Cystatin C ELISA Kit

E28L0567 96T
EUR 666.67

Rat Cystatin A ELISA Kit (CSTA)

RK04075 96 Tests
EUR 625.2

Rat Cystatin 3 ELISA Kit (CST3)

RK03595 96 Tests
EUR 625.2

Rat Cystatin (Cys)ELISA Kit

YLA1519RA-48T 48T
EUR 465

Rat Cystatin (Cys)ELISA Kit

YLA1519RA-96T 96T
EUR 600

Rabbit Cystatin C ELISA kit

E04C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cystatin C ELISA kit

E04C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cystatin C ELISA kit

E04C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cystatin C ELISA kit

E09C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cystatin C ELISA kit

E09C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cystatin C ELISA kit

E09C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Canine Cystatin C ELISA Kit

ELA-E0896d 96 Tests
EUR 1113.6

Rabbit Cystatin C ELISA Kit

ELA-E0896Rb 96 Tests
EUR 1113.6

Monkey Cystatin C ELISA Kit

IMNCSTCKT each
EUR 726
Description: Monkey Cystatin C ELISA Kit

Canine Cystatin C ELISA Kit

SL0101Ca - Ask for price

Porcine Cystatin C ELISA Kit

ELA-E0896p 96 Tests
EUR 1113.6

Cst8 ELISA Kit| Rat Cystatin-8 ELISA Kit

EF018523 96 Tests
EUR 826.8

Mouse Cys-C(Cystatin C) ELISA Kit

EM0065 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Mouse Cys-C (Cystatin C) ELISA Kit

EKE61607-5x96T 5x96T
EUR 2667.6

Mouse Cys-C (Cystatin C) ELISA Kit

EKE61607-96T 96T
EUR 561.6

Human Cys-C (Cystatin C) ELISA Kit

EKE60052-5x96T 5x96T
EUR 2667.6

Human Cys-C (Cystatin C) ELISA Kit

EKE60052-96T 96T
EUR 561.6

Monkey Cys-C(Cystatin C) ELISA Kit

EMK0019 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.094 ng/ml

Cys-C ELISA Kit| Mouse Cystatin C ELISA Kit

EF012850 96 Tests
EUR 826.8

Porcine Cys-C(Cystatin C) ELISA Kit

EP0046 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.375 ng/ml

Guinea pig Cystatin C ELISA kit

E05C0868-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Cystatin C ELISA kit

E05C0868-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Cystatin C ELISA kit

E05C0868-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human CST3(Cystatin C) ELISA Kit

EH0110 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human CST3(Cystatin C) ELISA Kit

EKF57027-48T 48T
EUR 396.9

Human CST3(Cystatin C) ELISA Kit

EKF57027-5x96T 5x96T
EUR 2693.25

Human CST3(Cystatin C) ELISA Kit

EKF57027-96T 96T
EUR 567

Human CST3(Cystatin C) ELISA Kit

E39EH0110 96T
EUR 595

Cys-C ELISA Kit| Monkey Cystatin C ELISA Kit

EF012693 96 Tests
EUR 826.8

Mouse Cystatin C ELISA kit (4X96T)

LF-EK50706 4×96T
EUR 2641.2

Human Cystatin C ELISA kit (4X96T)

LF-EK50566 4×96T
EUR 2641.2

Cystatin C (Human) ELISA Kit

EKA52036-5x96T 5x96T Ask for price

Cystatin C (Human) ELISA Kit

EKA52036-96T 96T
EUR 446.9

Rat Cystatin 3 (CST3) ELISA Kit

20-abx155418
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Cystatin 8 (CST8) ELISA Kit

abx391169-96tests 96 tests
EUR 1093.2

Rat Cystatin SN(CST1) ELISA kit

E02C2119-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cystatin SN(CST1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin SN(CST1) ELISA kit

E02C2119-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cystatin SN(CST1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin SN(CST1) ELISA kit

E02C2119-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cystatin SN(CST1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin SA(CST2) ELISA kit

E02C2121-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cystatin SA(CST2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin SA(CST2) ELISA kit

E02C2121-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cystatin SA(CST2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin SA(CST2) ELISA kit

E02C2121-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cystatin SA(CST2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Protecting impact of overexpression of PrxII on H2O2-induced cardiomyocyte harm

 Goal: Oxidative stress is likely one of the primary components resulting in myocardial cell harm, and the redox imbalance promotes apoptosis. Due to this fact, the aim of this research was to discover the protecting impact of PrxII on H2O2-induced H9c2 cell harm.

Supplies and strategies: The overexpressed PrxII cell mannequin was constructed by virus. The H9c2 cells had been handled with H2O2, and the supernatant and cells had been collected. Then, the chymotrypsin-like exercise, caspase-like exercise, and trypsin-like exercise had been detected by the package, and the expressions of P21, P27, and P53 had been detected by the ELISA methodology. Lastly, the expressions of antioxidant components, apoptosis-related components, and AMPK/Sirt1 signaling pathway had been detected by Western blot and Actual-time polymerase chain response (PCR).

Outcomes: Overexpression of PrxII inhibited the lower of enzyme exercise induced by H2O2, promoted the expressions of anti-oxidation components GPX1, GPX2, and GSX, and inhibited the expressions of apoptosis-related components P21, P27, and P53, and activated AMPK/Sirt1 pathway.

Metabolomic Approaches to Research Chemical Publicity-Associated Metabolism Alterations in Mammalian CellCultures

Natural organisms are repeatedly uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a gradual motion of stimuli-dependent variations.

  • The number of these chemical substances along with their concentrations contribute to the multiplicity of induced outcomes, along with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, as a result of the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and very adaptive to chemical stimuli.
  • Subsequently, studying the impression of endo- or xenobiotics over cell metabolism delivers priceless data to apprehend potential cell train of specific particular person molecules and think about their acute or energy benefits and toxicity.
  • The occasion of up to date metabolomics utilized sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now offers unprecedented choices for the speedy and atmosphere pleasant willpower of metabolic profiles of cells and additional difficult natural strategies.
  • Combined with the availability of well-established cell custom methods, these analytical methods appear fully suited to search out out the natural train and estimate the constructive and opposed outcomes of chemical substances in numerous cell kinds and fashions, even at hardly detectable concentrations.
  • Metabolic phenotypes shall be estimated from studying intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures current further entry to metabolites exchanged with progress media.
  • This textual content discusses analytical choices on the market for metabolic phenotyping of cell custom metabolism along with the general metabolomics workflow applicable for testing the natural train of molecular compounds.
  • We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the results of xenobiotics on cell metabolism.
  • We phrase that the concepts and techniques talked about primarily for xenobiotics publicity are extensively related to drug testing on the entire, along with endobiotics that cowl energetic metabolites, nutritional vitamins, peptides and proteins, cytokines, hormones, dietary nutritional vitamins, and so forth.

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