Evaluation of toxoplasmosis in pregnant women using dot-immunogold-silver

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Analysis of toxoplasmosis in pregnant girls utilizing dot-immunogold-silver staining with recombinant Toxoplasma gondii peroxiredoxin protein

 Background: Toxoplasma gondii an infection endangers human well being and impacts animal husbandry. Serological detection is the primary methodology used for epidemiological investigations and prognosis of toxoplasmosis. The important thing to efficient prognosis of toxoplasmosis is using a standardized antigen and a particular and delicate detection methodology. Peroxiredoxin is an antigenic protein and vaccine candidate antigen of T. gondii that has not but been exploited for diagnostic software.

Strategies: On this research, recombinant T. gondii peroxiredoxin protein (rTgPrx) was ready and utilized in dot-immunogold-silver staining (Dot-IGSS) to detect IgG antibodies in serum from mice and pregnant girls. The rTgPrx-Dot-IGSS methodology was established and optimized utilizing mouse serum. Moreover, serum samples from pregnant girls had been analyzed by rTgPrx-Dot-IGSS.

Outcomes: Forty serum samples from mice contaminated with T. gondii and twenty adverse serum samples had been analyzed. The sensitivity and specificity of rTgPrx-Dot-IGSS had been 97.5 and 100%, respectively, equal to these of a industrial ELISA package for anti-Toxoplasma IgG antibody. Moreover, 540 serum samples from pregnant girls had been screened with a industrial ELISA package. Eighty-three constructive and 60 adverse serum samples had been analyzed by rTgPrx-Dot-IGSS. The constructive price was 95.18%, akin to that obtained with the industrial ELISA package.

Conclusions: The Dot-IGSS methodology with rTgPrx as an antigen may be helpful for diagnosing T. gondii an infection in people.

The Validation of the Wheat Gluten ELISA Equipment.

Background: It is very important analyze the presence of wheat/gluten in meals to keep away from wheat allergy or celiac illness.

Goal: The Wheat/Gluten ELISA package was developed to measure complete wheat protein or gluten content material in wheat, barley, and rye cereals as uncooked supplies, and processed meals. Validation as as to if this package is appropriate for quantifying complete wheat protein/gluten was carried out.

Strategies: The Wheat/Gluten ELISA package was designed as a sandwich ELISA based mostly on antigliadin polyclonal antibody. Selectivity, interference research, matrix research together with incurred meals, robustness, stability, and lot-to-lot consistency research had been carried out for the Wheat/Gluten ELISA package. Incurred matrix research had been additionally carried out in an impartial laboratory.

Outcomes: The evaluation of 38 totally different substances revealed no cross-reactivity above the LOQ apart from oats. Recoveries of the spiked samples had been largely within the vary of 75-140%, together with an impartial laboratory outcome. The LOD of the ELISA was discovered to be 0.02-0.16 mg/kg. Robustness testing proved that extraction time and incubation time of first response and enzyme response had no important affect on quantified worth. The steadiness at 2-8°C was discovered to exceed 12 months. Good lot-to-lot consistency was noticed.

Conclusions: The Wheat/Gluten ELISA package confirmed good analytical efficiency within the quantitative evaluation of complete wheat protein/gluten within the recognized meals merchandise utilizing the AOAC Efficiency Examined Methodology(s)SM program.

Highlights: The Wheat/Gluten ELISA package was validated and confirmed good analytical efficiency within the quantitative evaluation of complete wheat protein/gluten in meals.

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Rat Cystatin C ELISA Kit

IRTCSTCKT each
EUR 702
Description: Rat Cystatin C ELISA Kit

Rat Cystatin C ELISA Kit

MBS763996-10x96StripWells 10x96-Strip-Wells
EUR 3900

Rat Cystatin C ELISA Kit

MBS763996-48StripWells 48-Strip-Wells
EUR 340

Rat Cystatin C ELISA Kit

MBS763996-5x96StripWells 5x96-Strip-Wells
EUR 2045

Rat Cystatin C ELISA Kit

MBS763996-96StripWells 96-Strip-Wells
EUR 455

Rat Cystatin C ELISA Kit

MBS733592-10x96StripWells 10x96-Strip-Wells
EUR 5685

Rat Cystatin C ELISA Kit

MBS733592-48StripWells 48-Strip-Wells
EUR 485

Rat Cystatin C ELISA Kit

MBS733592-5x96StripWells 5x96-Strip-Wells
EUR 3020

Rat Cystatin C ELISA Kit

MBS733592-96StripWells 96-Strip-Wells
EUR 690

Rat Cystatin C ELISA Kit

MBS731876-10x96StripWells 10x96-Strip-Wells
EUR 5685

Rat Cystatin C ELISA Kit

MBS731876-48StripWells 48-Strip-Wells
EUR 485

Rat Cystatin C ELISA Kit

MBS731876-5x96StripWells 5x96-Strip-Wells
EUR 3020

Rat Cystatin C ELISA Kit

MBS731876-96StripWells 96-Strip-Wells
EUR 690

Rat Cystatin C ELISA Kit

MBS824495-5x96Tests 5x96Tests
EUR 2880

Rat Cystatin C ELISA Kit

MBS824495-96Tests 96Tests
EUR 645

Rat Cystatin C ELISA Kit

MBS8421406-1Kit 1Kit
EUR 755

Rat Cystatin C ELISA Kit

MBS8421406-5x1Kit 5x1Kit
EUR 3455

Rat Cys-C(Cystatin C) ELISA Kit

EKF58465-48T 48T
EUR 396.9

Rat Cys-C(Cystatin C) ELISA Kit

EKF58465-5x96T 5x96T
EUR 2693.25

Rat Cys-C(Cystatin C) ELISA Kit

EKF58465-96T 96T
EUR 567

Rat Cys-C (Cystatin C) ELISA Kit

EKE62085-5x96T 5x96T
EUR 2667.6

Rat Cys-C (Cystatin C) ELISA Kit

EKE62085-96T 96T
EUR 561.6

Rat Cys-C(Cystatin C) ELISA Kit

ELK9629-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat Cys-C(Cystatin C) ELISA Kit

ELK9629-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat Cys-C(Cystatin C) ELISA Kit

ER0891 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 9.375pg/ml

Rat Cys-C(Cystatin C) ELISA Kit

RE3377R-48wells 48 wells
EUR 125.55

Rat Cys-C(Cystatin C) ELISA Kit

RE3377R-96wells 96 wells
EUR 179.55

Rat Cys-C (Cystatin C) ELISA Kit

MBS7615557-10x96StripWells 10x96-Strip-Wells
EUR 3900

Rat Cys-C (Cystatin C) ELISA Kit

MBS7615557-48StripWells 48-Strip-Wells
EUR 340

Rat Cys-C (Cystatin C) ELISA Kit

MBS7615557-5x96StripWells 5x96-Strip-Wells
EUR 2045

Rat Cys-C (Cystatin C) ELISA Kit

MBS7615557-96StripWells 96-Strip-Wells
EUR 455

Rat Cys-C (Cystatin C) ELISA Kit

MBS8819875-10x96StripWells 10x96-Strip-Wells
EUR 3130

Rat Cys-C (Cystatin C) ELISA Kit

MBS8819875-48StripWells 48-Strip-Wells
EUR 350

Rat Cys-C (Cystatin C) ELISA Kit

MBS8819875-5x96StripWells 5x96-Strip-Wells
EUR 1710

Rat Cys-C (Cystatin C) ELISA Kit

MBS8819875-96StripWells 96-Strip-Wells
EUR 445

Rat Cys-C (Cystatin C) ELISA Kit

MBS2508986-10x96Tests 10x96Tests
EUR 3265

Rat Cys-C (Cystatin C) ELISA Kit

MBS2508986-24Tests 24Tests
EUR 225

Rat Cys-C (Cystatin C) ELISA Kit

MBS2508986-48Tests 48Tests
EUR 350

Rat Cys-C (Cystatin C) ELISA Kit

MBS2508986-5x96Tests 5x96Tests
EUR 1655

Rat Cys-C (Cystatin C) ELISA Kit

MBS2508986-96Tests 96Tests
EUR 395

Rat Cystatin-C ELISA Kit

EK2416 96 tests
EUR 511

Rat Cystatin-C ELISA Kit

MBS9426256-5x96Tests 5x96Tests
EUR 2465

Rat Cystatin-C ELISA Kit

MBS9426256-96Tests 96Tests
EUR 540

Rat Cystatin-C ELISA Kit

MBS2880855-10x96StripWells 10x96-Strip-Wells
EUR 3290

Rat Cystatin-C ELISA Kit

MBS2880855-48StripWells 48-Strip-Wells
EUR 340

Rat Cystatin-C ELISA Kit

MBS2880855-5x96StripWells 5x96-Strip-Wells
EUR 1800

Rat Cystatin-C ELISA Kit

MBS2880855-96StripWells 96-Strip-Wells
EUR 435

Rat Cystatin C ELISA

1042 -
EUR 567
Description: Nephrology ELISA

Rat Cystatin C ELISA

GWB-374Z42 96 well Ask for price

Cystatin C ELISA Kit (Rat) (OKIA00140)

OKIA00140 96 Wells
EUR 756
Description: Description of target: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L. ;Species reactivity: ;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.206 ng/ml

Rat Cystatin C,Cys-C ELISA kit

E02C2122-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin C,Cys-C ELISA kit

E02C2122-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin C,Cys-C ELISA kit

E02C2122-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cystatin C (Cys-C) ELISA Kit

EKN44675-48T 48T
EUR 370.02

Rat Cystatin C (Cys-C) ELISA Kit

EKN44675-5x96T 5x96T
EUR 2510.85

Rat Cystatin C (Cys-C) ELISA Kit

EKN44675-96T 96T
EUR 528.6

Rat Cystatin C(Cys-C) Elisa Kit

EK720197 96 Wells
EUR 0.54

Rat Cystatin C,Cys-C ELISA Kit

EKC38930-96T 96T
EUR 765.7

Rat Cystatin C,Cys-C ELISA Kit

EKC38930-48T 48T
EUR 535.99

Rat Cystatin C,Cys-C ELISA Kit

EKC38930-5x96T 5x96T
EUR 3637.08

Rat Cystatin C (Cys-C) ELISA Kit

EKU03564-48T 48T
EUR 542.78

Rat Cystatin C (Cys-C) ELISA Kit

EKU03564-5x96T 5x96T
EUR 3683.15

Rat Cystatin C (Cys-C) ELISA Kit

EKU03564-96T 96T
EUR 775.4

Rat Cystatin C,Cys-C ELISA Kit

E01A12356 96T
EUR 700
Description: ELISA

Rat Cystatin C, Cys-C ELISA Kit

CSB-E08385r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Cystatin C, Cys-C ELISA Kit

1-CSB-E08385r
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Cystatin C,Cys-C ELISA Kit

CN-01845R1 96T
EUR 577.2

Rat Cystatin C,Cys-C ELISA Kit

CN-01845R2 48T
EUR 398.4

Rat Cystatin C,Cys-C ELISA Kit

YLA0395RA-48T 48T Ask for price

Rat Cystatin C,Cys-C ELISA Kit

YLA0395RA-96T 96T Ask for price

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-48Tests 48 Tests Ask for price

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-96Tests 96 Tests
EUR 500

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-48T 48T
EUR 424.8

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-96T 96T
EUR 685.2

Rat Cystatin C(Cys-C)  ELISA Kit

NSL1529r 96 Tests
EUR 498

Rat Cystatin C(Cys-C)ELISA Kit

QY-E11499 96T
EUR 433.2

Rat Cystatin C,Cys-C ELISA Kit

SL0221Ra -
EUR 498

Rat Cystatin C,Cys-C ELISA KIT

E0145Ra-1096T 10*96T
EUR 4122

Rat Cystatin C,Cys-C ELISA KIT

E0145Ra-48wells 48 wells
EUR 300

Rat Cystatin C,Cys-C ELISA KIT

E0145Ra-596T 5*96T
EUR 2061

Rat Cystatin C,Cys-C ELISA KIT

E0145Ra-96wells 96 wells
EUR 458

Rat Cystatin C, Cys-C ELISA Kit

MBS704068-10x96StripWells 10x96-Strip-Wells
EUR 5575

Rat Cystatin C, Cys-C ELISA Kit

MBS704068-24StripWellsLIMIT1 24-Strip-Wells(LIMIT1)
EUR 275

Rat Cystatin C, Cys-C ELISA Kit

MBS704068-48StripWells 48-Strip-Wells
EUR 545

Rat Cystatin C, Cys-C ELISA Kit

MBS704068-5x96StripWells 5x96-Strip-Wells
EUR 2920

Rat Cystatin C, Cys-C ELISA Kit

MBS704068-96StripWells 96-Strip-Wells
EUR 765

Rat Cystatin C, Cys-C ELISA Kit

MBS7255040-10x96StripWells 10x96-Strip-Wells
EUR 5685

Rat Cystatin C, Cys-C ELISA Kit

MBS7255040-48StripWells 48-Strip-Wells
EUR 485

Rat Cystatin C, Cys-C ELISA Kit

MBS7255040-5x96StripWells 5x96-Strip-Wells
EUR 3020

Rat Cystatin C, Cys-C ELISA Kit

MBS7255040-96StripWells 96-Strip-Wells
EUR 690

Rat Cystatin C, Cys-C ELISA Kit

MBS160516-10x96StripWells 10x96-Strip-Wells
EUR 3955

Rat Cystatin C, Cys-C ELISA Kit

MBS160516-48StripWells 48-Strip-Wells
EUR 305

Rat Cystatin C, Cys-C ELISA Kit

MBS160516-5x96StripWells 5x96-Strip-Wells
EUR 2005

Rat Cystatin C, Cys-C ELISA Kit

MBS160516-96StripWells 96-Strip-Wells
EUR 475

Rat Cystatin C (Cys-C) ELISA Kit

MBS3807762-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Cystatin C (Cys-C) ELISA Kit

MBS3807762-48StripWells 48-Strip-Wells
EUR 550

Rat Cystatin C (Cys-C) ELISA Kit

MBS3807762-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Cystatin C (Cys-C) ELISA Kit

MBS3807762-96StripWells 96-Strip-Wells
EUR 765

Rat Cystatin C (Cys-C) ELISA Kit

MBS287276-10x96StripWells 10x96-Strip-Wells
EUR 6255

Rat Cystatin C (Cys-C) ELISA Kit

MBS287276-48StripWells 48-Strip-Wells
EUR 445

Rat Cystatin C (Cys-C) ELISA Kit

MBS287276-5x96StripWells 5x96-Strip-Wells
EUR 3290

Rat Cystatin C (Cys-C) ELISA Kit

MBS287276-96StripWells 96-Strip-Wells
EUR 730

Rat Cystatin C (Cys-C) ELISA Kit

MBS265782-10x96StripWells 10x96-Strip-Wells
EUR 3290

Rat Cystatin C (Cys-C) ELISA Kit

MBS265782-48StripWells 48-Strip-Wells
EUR 295

Rat Cystatin C (Cys-C) ELISA Kit

MBS265782-5x96StripWells 5x96-Strip-Wells
EUR 1800

Rat Cystatin C (Cys-C) ELISA Kit

MBS265782-96StripWells 96-Strip-Wells
EUR 450

Rat Cystatin C(Cys-C) ELISA Kit

EIA05552r each
EUR 480

Nori® Rat Cystatin C ELISA Kit

GR118348 96-well
EUR 461

Rat Cystatin C (CST3) ELISA Kit

abx256140-96tests 96 tests
EUR 801.6

Rat Cystatin C (CST3) ELISA Kit

abx256859-96tests 96 tests
EUR 801.6

Protecting impact of overexpression of PrxII on H2O2-induced cardiomyocyte harm

 Goal: Oxidative stress is likely one of the primary components resulting in myocardial cell harm, and the redox imbalance promotes apoptosis. Due to this fact, the aim of this research was to discover the protecting impact of PrxII on H2O2-induced H9c2 cell harm.

Supplies and strategies: The overexpressed PrxII cell mannequin was constructed by virus. The H9c2 cells had been handled with H2O2, and the supernatant and cells had been collected. Then, the chymotrypsin-like exercise, caspase-like exercise, and trypsin-like exercise had been detected by the package, and the expressions of P21, P27, and P53 had been detected by the ELISA methodology. Lastly, the expressions of antioxidant components, apoptosis-related components, and AMPK/Sirt1 signaling pathway had been detected by Western blot and Actual-time polymerase chain response (PCR).

Outcomes: Overexpression of PrxII inhibited the lower of enzyme exercise induced by H2O2, promoted the expressions of anti-oxidation components GPX1, GPX2, and GSX, and inhibited the expressions of apoptosis-related components P21, P27, and P53, and activated AMPK/Sirt1 pathway.

Metabolomic Approaches to Research Chemical Publicity-Associated Metabolism Alterations in Mammalian CellCultures

Natural organisms are repeatedly uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a gradual motion of stimuli-dependent variations.

  • The number of these chemical substances along with their concentrations contribute to the multiplicity of induced outcomes, along with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, as a result of the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and very adaptive to chemical stimuli.
  • Subsequently, studying the impression of endo- or xenobiotics over cell metabolism delivers priceless data to apprehend potential cell train of specific particular person molecules and think about their acute or energy benefits and toxicity.
  • The occasion of up to date metabolomics utilized sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now offers unprecedented choices for the speedy and atmosphere pleasant willpower of metabolic profiles of cells and additional difficult natural strategies.
  • Combined with the availability of well-established cell custom methods, these analytical methods appear fully suited to search out out the natural train and estimate the constructive and opposed outcomes of chemical substances in numerous cell kinds and fashions, even at hardly detectable concentrations.
  • Metabolic phenotypes shall be estimated from studying intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures current further entry to metabolites exchanged with progress media.
  • This textual content discusses analytical choices on the market for metabolic phenotyping of cell custom metabolism along with the general metabolomics workflow applicable for testing the natural train of molecular compounds.
  • We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the results of xenobiotics on cell metabolism.
  • We phrase that the concepts and techniques talked about primarily for xenobiotics publicity are extensively related to drug testing on the entire, along with endobiotics that cowl energetic metabolites, nutritional vitamins, peptides and proteins, cytokines, hormones, dietary nutritional vitamins, and so forth.

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